ME/CFS RESEARCH FORUM: 26-27 March 2007
University of Adelaide
Convenor: Alison Hunter Memorial Foundation
Daniel L. Peterson1 , David Pomeranz2, Kathryn Hagen2, Byron Hsu2, Judy A. Mikovits2 and Francis W. Ruscetti3 1 Sierra Internal Medicine and 2Simaron, LLC, 865 Tahoe Blvd Incline Village, NV Lab of Leukocyte Biology, CCR, National Cancer Institute, Frederick, MD.
Microarray Analysis of Mammalian virus expression in a Cohort of CFS patients with Clonal TCR-y and Lymphoid Malignancies.
Patients with chronic fatigue syndrome (CFS) have similar immune aberrations including, low NK activity, oligoclonal expansions of T cells, and activation of 2-5A synthetase and RNase L antiviral pathway. We have previously reported a significant incidence of CFS patients with a dysregulated RNaseL pathway develop monoclonal TCR -g rearrangements and mantle cell lymphoma (MCL). Because RNAseL is an important effector of the innate immune response and clonal TCR-g rearrangements are detected in certain persistent viral infections, we hypothesized that chronic inflammatory stimulation from low level active and recurrent infections leads to the pathogenesis characterized by CFS with increased incidence of rare lymphoma. To investigate this hypothesis we employed a Viral Detection DNA microarray composed of more than 10,000 oligonucleotides that can detect all known mammalian and avian pathogenic viruses. Each virus is represented by 10 or more features, (both conserved and unique regions represented), to provide redundancy and also to allow the identification of new viruses that may arise via recombination. Human influenza and avian flu viruses are represented as tiling paths to aid in the identification of viral sub-types as well as the precise definition of influenza recombination events. Both RNA and DNA viruses are represented. The technology is highly sensitive detecting DNA virus expression as low as 5-10 virus particles and RNA viruses at a sensitivity of 20-200 virus particles. 32 patient samples were analyzed on the array 14 of those with clonal TCR-g rearrangements and 5with MCL. Results will be discussed.
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